Solid-phase enzyme-linked immunosorbent assay (direct option) for detecting toxigenic vibrio cholerae serogroups О1 and О139

As a result of the studies, method direct solid-phase enzyme immunoassay (ELISA test) was optimized for detecting toxigenic strains Vibrio cholerae O1 and O139 serogroups based on monoclonal polyclonal antitoxic immunoglobulin peroxidase conjugates. The study detected following optimal parameters analysis: sensitization tablet by toxicodermia drugs diluted in phosphate-saline buffer (PBS) pH 7.4, up to titers 1:2-1:4, their adsorption solid phase 2 hours at 37° C, blocking free binding sites 1% solution bovine serum albumin (BSA) 30 minutes (at 37°C); contact time investigated sample with mono- or conjugates - 37°C). Tests homologous heterologous showed high specificity possibility its use cholerae.